Play as Archer - Successfully complete Rin's storyline to unlock Archer.
Play as Assassin - Successfully complete Lancer's storyline to unlock Assassin.

Play as Bazett - Successfully complete Taiga's storyline to unlock Bazett.
Play as Berserker - Successfully complete Saber's storyline to unlock Berserker.

Play as Caren - Successfully complete Taiga's storyline to unlock Caren.

Play as Dark Saber - Successfully complete Berserker's storyline to unlock Dark Saber.
Play as Dark Sakura - Successfully complete Ilya's storyline to unlock Dark Sakura.

Play as Gilgamesh - Successfully complete Dark Saber's storyline to unlock Gilgamesh.

Play as Ilya - Successfully complete Shiro's storyline to unlock Ilya.

Play as Kirei - Successfully complete Gilgamesh's storyline to unlock Kirei.

Play as Shinji - Successfully complete Sakura's storyline to unlock Shinji.
Play as Soichiro - Successfully complete Caster's storyline to unlock Soichiro.

Play as Taiga - Successfully complete Kirei's storyline to unlock Taiga.
Play as True Assassin - Successfully complete Rider's storyline to unlock True Assassin.

Alternate costumes - Successfully complete the indicated task to unlock the corresponding alternate costume:

Archer's casual wear: Successfully complete Archer's storyline on the Hard difficulty setting.

Caster's casual wear: Successfully complete Caster's storyline on the Hard difficulty setting.

Gilgamesh's casual wear: Successfully complete Gilgamesh's storyline on the Hard difficulty setting.

Ilya's sports wear: Successfully complete Ilya's storyline on the Hard difficulty setting.

Lancer's casual wear: Successfully complete Lancer's storyline on the Hard difficulty setting.

Rider's casual wear: Successfully complete Rider's storyline on the Hard difficulty setting.

Rin's school uniform: Successfully complete Rin's storyline on the Hard difficulty setting.

Saber's casual wear: Successfully complete Saber's storyline on the Hard difficulty setting.

Saber's lion costume: Successfully complete Archer's storyline on the Normal difficulty setting.

Sakura's casual wear: Successfully complete Sakura's storyline on the Hard difficulty setting.

Sakura's princess dress: Successfully complete Shinji's storyline on the Normal difficulty setting.

Shiro's casual wear: Successfully complete Shiro's storyline on the Hard difficulty setting.

Taiga's Kendo wear: Successfully complete Taiga's storyline on the Hard difficulty setting.