Note: This game is also titled Naruto Shippuuden: Gekitou Ninja Taisen EX.

Play as Deidara - Successfully complete Story mode to unlock Deidara.

Play as Itachi - Successfully complete Score Attack mode with Kakashi to unlock Itachi.

Play as Kankurou - Successfully complete Story mode to unlock Kankurou.

Play as Kisame - Successfully complete Score Attack mode with Guy Might to unlock Kisame.

Play as Sasori - Successfully complete Score Attack mode with all characters to unlock Sasori.

Play as Temari - Successfully complete Story mode to unlock Temari.

Play as Ten Ten - Successfully complete Score Attack mode with Rock Lee to unlock Ten Ten.

Silent Shrine stage - Successfully complete Story mode to unlock Silent Shrine stage.

Valley Of The End stage - Successfully complete Story mode and unlock all characters to unlock Valley Of The End stage.